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high capacity cdna reverse transcription kit  (TaKaRa)


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    Structured Review

    TaKaRa high capacity cdna reverse transcription kit
    High Capacity Cdna Reverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+capacity+cdna+reverse+transcription+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pmc13132331-74-7-12
    Average 99 stars, based on 14654 article reviews
    high capacity cdna reverse transcription kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Heat shock protein 70 regulates m6A modification in response to heat shock in esophageal squamous cell carcinoma.
    Article Snippet: Heat shock has been known to induce hyperplasia in esophageal epithelial cells.. It is widely considered as a crucial risk factor in the initiation and development of esophageal squamous cell carcinoma (ESCC), yet our understanding of the underlying mechanisms remains limited.. The m6A modification of mRNA plays a role in mediating several cellular processes and is critical during cell stress.

    Article Title: DCBLD1 as a novel prognostic biomarker and therapeutic target in breast cancer: clinical validation and mechanistic insights
    Article Snippet: Total RNA was extracted with TRIzol reagent (Tiangen Biotech (Beijing) Co., Ltd., China). .. Complementary DNA (cDNA) was synthesized with a high-capacity cDNA reverse transcription kit (TaKaRa, Cat. #RR047A). .. Quantitative reverse transcription PCR (qRT-PCR) was performed on Applied Biosystems 7500 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix kits (Takara Biotechnology (Dalian) Co., Ltd., China, Cat. #RR820A).

    Article Title: 4EBP1 phosphorylation mediates TGF-β1-induced hepatic fibrosis and upregulates HIF-1α expression
    Article Snippet: Cell Counting Kit-8 , Share-bio , SB-CCK8. .. High-Capacity cDNA Reverse Transcription Kit , Takara Bio , RR036A. .. TBGreenTM Premix Ex TaqTM II , Takara Bio , Tli RNaseH Plus.

    Article Title: Glycyrrhetinic acid augments lipid catabolism via immune-neural modulation in adipose tissue.
    Article Snippet: RNA concentration was measured using a NanoDrop microvolume spectrophotometer (Thermo Fisher Scientific, #701–058112). .. Subsequently, 0.5 lg of RNA was reverse transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Takara, #RR036A). .. RT-PCR was performed with Universal SYBR Green Supermix (Bio-Rad Laboratories, #1725121) on a CFX96 RealTime PCR Detection System v.2.1 (Bio-Rad Laboratories).

    Article Title: SEC62 at mitochondria-associated membranes drives MASH progression by suppressing ATAD3B-mediated mitochondrial quality control.
    Article Snippet: Background: The progression of metabolic dysfunction-associated steatohepatitis (MASH) involves chronic, irreversible inflammatory responses linked to intracellular organelle dysfunction.. While endoplasmic reticulum (ER) stress and mitochondrial impairment are recognized as critical drivers, the precise molecular mechanisms governing inter-organelle communication in this disease context remain incompletely understood.. Aim: This study aimed to investigate the role of the ER transmembrane protein SEC62 in MASH pathogenesis.

    Article Title: Evogliptin prevents ceramide-induced pyroptosis during calcification via modulation of NLRP3/GSDM-D mediated pathway in Vascular Smooth Muscle Cells
    Article Snippet: .. RNA was treated with DNase I and used for the reverse transcription using the high-capacity cDNA reverse transcription kit (PrimeScriptTM Ist strand cDNA Synthesis Kit (TaKaRa Bio USA, Inc.). ..

    Article Title: Lactobacillus rhamnosus CMU-pb-7 Alleviates Diabetic Nephropathy in a High-Fat Diet-Streptozotocin Rat Model
    Article Snippet: [24]. qPCR Following the protocol of our prior study [25], total RNA was isolated from rat renal tissue with Trizol reagent (15596026,Thermo Fisher, Waltham, USA), with its concentration quantified using Nanodrop 2000. .. Reverse transcription was performed using 500 ng of total RNA in a thermal cycler (Mastercycler, Eppendorf, Hamburg, Germany), utilizing a high-capacity cDNA reverse transcription kit (RR047A, Takara, Shiga, Japan). qPCR was conducted in a Real-Time PCR System (QuantStudio 5, Applied Biosystems, Thermo Fisher, Waltham, USA)) using TB Green Premix Ex Taq (RR420A, Takara, Shiga, Japan).The mRNA expression of β-actin was applied as the internal reference and the data were analyzed by 2−ΔΔCT method for relative quantification. ..

    Synthesized:

    Article Title: DCBLD1 as a novel prognostic biomarker and therapeutic target in breast cancer: clinical validation and mechanistic insights
    Article Snippet: Total RNA was extracted with TRIzol reagent (Tiangen Biotech (Beijing) Co., Ltd., China). .. Complementary DNA (cDNA) was synthesized with a high-capacity cDNA reverse transcription kit (TaKaRa, Cat. #RR047A). .. Quantitative reverse transcription PCR (qRT-PCR) was performed on Applied Biosystems 7500 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix kits (Takara Biotechnology (Dalian) Co., Ltd., China, Cat. #RR820A).

    Article Title: SEC62 at mitochondria-associated membranes drives MASH progression by suppressing ATAD3B-mediated mitochondrial quality control.
    Article Snippet: Background: The progression of metabolic dysfunction-associated steatohepatitis (MASH) involves chronic, irreversible inflammatory responses linked to intracellular organelle dysfunction.. While endoplasmic reticulum (ER) stress and mitochondrial impairment are recognized as critical drivers, the precise molecular mechanisms governing inter-organelle communication in this disease context remain incompletely understood.. Aim: This study aimed to investigate the role of the ER transmembrane protein SEC62 in MASH pathogenesis.

    cDNA Synthesis:

    Article Title: Evogliptin prevents ceramide-induced pyroptosis during calcification via modulation of NLRP3/GSDM-D mediated pathway in Vascular Smooth Muscle Cells.
    Article Snippet: .. RNA was treated with DNase I and used for the reverse transcription using the high-capacity cDNA reverse transcription kit (PrimeScriptTM Ist strand cDNA Synthesis Kit (TaKaRa Bio USA, Inc.). ..

    Article Title: Evogliptin prevents ceramide-induced pyroptosis during calcification via modulation of NLRP3/GSDM-D mediated pathway in Vascular Smooth Muscle Cells
    Article Snippet: .. RNA was treated with DNase I and used for the reverse transcription using the high-capacity cDNA reverse transcription kit (PrimeScriptTM Ist strand cDNA Synthesis Kit (TaKaRa Bio USA, Inc.). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Lactobacillus rhamnosus CMU-pb-7 Alleviates Diabetic Nephropathy in a High-Fat Diet-Streptozotocin Rat Model
    Article Snippet: [24]. qPCR Following the protocol of our prior study [25], total RNA was isolated from rat renal tissue with Trizol reagent (15596026,Thermo Fisher, Waltham, USA), with its concentration quantified using Nanodrop 2000. .. Reverse transcription was performed using 500 ng of total RNA in a thermal cycler (Mastercycler, Eppendorf, Hamburg, Germany), utilizing a high-capacity cDNA reverse transcription kit (RR047A, Takara, Shiga, Japan). qPCR was conducted in a Real-Time PCR System (QuantStudio 5, Applied Biosystems, Thermo Fisher, Waltham, USA)) using TB Green Premix Ex Taq (RR420A, Takara, Shiga, Japan).The mRNA expression of β-actin was applied as the internal reference and the data were analyzed by 2−ΔΔCT method for relative quantification. ..

    Expressing:

    Article Title: Lactobacillus rhamnosus CMU-pb-7 Alleviates Diabetic Nephropathy in a High-Fat Diet-Streptozotocin Rat Model
    Article Snippet: [24]. qPCR Following the protocol of our prior study [25], total RNA was isolated from rat renal tissue with Trizol reagent (15596026,Thermo Fisher, Waltham, USA), with its concentration quantified using Nanodrop 2000. .. Reverse transcription was performed using 500 ng of total RNA in a thermal cycler (Mastercycler, Eppendorf, Hamburg, Germany), utilizing a high-capacity cDNA reverse transcription kit (RR047A, Takara, Shiga, Japan). qPCR was conducted in a Real-Time PCR System (QuantStudio 5, Applied Biosystems, Thermo Fisher, Waltham, USA)) using TB Green Premix Ex Taq (RR420A, Takara, Shiga, Japan).The mRNA expression of β-actin was applied as the internal reference and the data were analyzed by 2−ΔΔCT method for relative quantification. ..

    Quantitative Proteomics:

    Article Title: Lactobacillus rhamnosus CMU-pb-7 Alleviates Diabetic Nephropathy in a High-Fat Diet-Streptozotocin Rat Model
    Article Snippet: [24]. qPCR Following the protocol of our prior study [25], total RNA was isolated from rat renal tissue with Trizol reagent (15596026,Thermo Fisher, Waltham, USA), with its concentration quantified using Nanodrop 2000. .. Reverse transcription was performed using 500 ng of total RNA in a thermal cycler (Mastercycler, Eppendorf, Hamburg, Germany), utilizing a high-capacity cDNA reverse transcription kit (RR047A, Takara, Shiga, Japan). qPCR was conducted in a Real-Time PCR System (QuantStudio 5, Applied Biosystems, Thermo Fisher, Waltham, USA)) using TB Green Premix Ex Taq (RR420A, Takara, Shiga, Japan).The mRNA expression of β-actin was applied as the internal reference and the data were analyzed by 2−ΔΔCT method for relative quantification. ..



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